![]() This desirable effect was achieved without increasing the doses of individual nutrients in the mixture 1. These nutrients used together could further decrease cancer cell growth by up to 65% moreover, the complete inhibition of cancer cell growth (100%) could be achieved by including small amounts of a few other micronutrients. Help to maintain balanced cellular metabolism.įor example, we observed that vitamin C, lysine, proline and green tea extract, when used individually, were able to decrease cancer cell growth by 26% each.Eliminate the need for huge doses of each nutrient and increase bioavailability of other nutrients.Maximize the efficacy of each individual component in the combination.Help preserve the nutrients through biological recycling – nutrient synergy is an economic way to generate energy in our body’s cells.Our many years of scientific research have confirmed that the most effective micronutrient supplementation can be achieved by applying specific and properly selected micronutrient combinations acting in biological synergy. This is often the reason why some clinical studies using a single compound or a random combination of a few nutrients bring inconsistent results. ![]() Supplementing with a very large dose of any one nutrient may worsen the deficiency of others, and the use of random combinations of nutrients may with time create a metabolic imbalance in the body. Their optimal interaction and cellular balance is the basis of health. All nutrients participate in complex metabolic processes in our body and interact with other nutrients either by enhancing or opposing their action. Vitamins, minerals and other micronutrients are required for the normal functions of our body, and they do not work alone. Based on the proper selection and correct combinations of micronutrients, thus maximizing their desired biological impact, we call it “micronutrient synergy”. Our research has introduced a new principle in modern nutrition. ![]() Especially considering that most of the nutritional supplements you find on the store shelves are either individual compounds or randomly combined ingredients, often selected according to marketing trends. All rights reserved.Selecting the most effective supplementation is not an easy task. Overall, Sal and 1,25D act synergistically, inhibiting cell proliferation by activating simultaneously multiple death pathways and may be a novel and promising luminal A breast cancer therapy strategy.ġ,25 Dihydroxyvitamin D(3) Autophagy Breast cancer Mammosphere Paraptosis.Ĭopyright © 2022 Elsevier Inc. ![]() Extensive cytoplasmic vacuolization, a morphological characteristic found in paraptosis, was also seen and could be triggered by endoplasmic reticulum stress (ER) as we found transcriptional upregulation of genes related to ER stress (ATF6, GADD153, GADD45G, EIF2AK3, and HSPA5). We observed the induction of cell death by apoptosis with upregulation in mRNA levels of apoptosis-related genes (CASP7, CASP9, and BBC3). ![]() We report that Sal and 1,25D act synergistically in MCF-7 mammospheres and monolayer causing G1 cell cycle arrest, reduction of mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) overproduction with a long-lasting cytotoxic response represented by clonogenic and mammosphere assay. We also aim to evaluate the molecular mechanism of Sal and 1,25D-mediated effects. This study aims to examine the cytotoxic and antiproliferative effect of Sal associated with 1,25D on MCF-7 breast carcinoma cell line cultured in monolayer (2D) and three-dimensional models (mammospheres). Salinomycin (Sal) has anticancer activity against cancer cell lines. 1α, 25, dihydroxyvitamin D 3 (1,25D), the active form of vitamin D 3, has antitumor properties in several cancer cell lines in vitro. ![]()
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